Example protocols for easy transfer of established methods to the AmpliGrid system.
- Singleplex or Multiplex PCR of human DNA on AmpliGrid AG480F
In the singleplex PCR system, one DNA fragment is amplified using human template and the multiplex PCR kit from Qiagen or Promega GoTaq DNA Polymerase together with AmpliGrid slides.
In the multiplex PCR system, four DNA fragments are amplified using female human DNA template and six DNA fragments are amplified using male human template and the multiplex PCR kit from Qiagen or Promega GoTaq DNA Polymerase together with AmpliGrid slides.
(Download pdf / 53 KB) - Plasmid Amplification and Sequencing on AmpliGrid AG480F
This protocol describes the amplification of plasmid DNA and the following sequencing reaction directly on the AmpliGrid Slide. (Download pdf / 57 KB) - On-Slide DNA Sequencing using AmpliGrid AG480F
This protocol describes the sequencing procedure after PCR of human DNA directly on the AmpliGrid slide. (Download pdf / 50 KB) - RT-PCR on total RNA and single cells using AmpliGrid AG480F
This process instruction describes the processing of the RT-PCR for human lymphocytes or human total RNA. (Download pdf / 47 KB) - Genetic Profiling with Applied Biosystems AmpFISTR SEfiler on AmpliGrid AG480F
In this PCR system, 11 specific STR loci are amplified using human DNA as template and the AmpFISTR SEfiler kit from Applied Biosystems together with AmpliGrid slides. (Download pdf / 53 KB) - Genetic Profiling with Promega PowerPlex 16 on AmpliGrid AG480F
This protocol describes the process of amplifying STR DNA fragments using the Promega PowerPlex 16 System. (Download pdf / 50 KB)
